Screening Transcript Associated with V-CMS Wheat Using cDNA-RAPD Analysis

In order to study the mechanism of V-type CMS wheat,a set of near isogenic lines of restoring gene to wheat V-CMS were used as materials,the mtRNAs of male sterile line,maintainer line and F1 hybrid were compared by cDNA-RAPD method.Two hundred and eighty-four primers were screened,104 of them were polymorphic between male sterile line and maintainer line,and 22 primers reuealed polymorphisms between male sterility line and F1 hybrid.A fragment of S238999 specifically expressed in cytoplasm male sterility line,was proved to be differentially expressed between cytoplasmic male sterility line with maintainer line and F1 hybrid by Northern hybridization,its transcripts abundance greatly reduced in fertility-restored line containing Rf.It was inferred that this fragment might be relative to V-CMS.Homology analysis showed that there existed 84 bp homologous sequences between a Hordeum vulgare genomic DNA fragment and S238999,this fragment may be a newly discovered sequence related to wheat V-CMS.