RAPD Analysis of Mitochondrial DNAs of Cytoplasmic Male Sterile Line and Its Maintainer in Tobacco(Nicotiana tobacum)

The mitochondrial DNA of 2 CMS tobacco lines and their homolog maintainer lines were analysed by the method of random amplified polymorphic DNA using 124 random 10-base primers.The result showed that 112 primers, 92.56% of total primers,did not amplified ploymorphic band between CMS and maintainer lines.This indicated the homolog of mitochondria between CMS and maintainer line.Meanwhile,some obvious differences in mitochondrial genome were detected between CMS and maintainer line.The relationship of these different bands in CMS to male sterility needs farther study.