RAPD analysis for mitochondrial DNAs of the BT cytoplasmic male sterile (cms) line and its associated lines in rice (Oryza sativa)

BT type rice cytoplasmic male sterility line were compared with its maintainer,restorer and F 1 hybrid using mitochondrial DNA (mtDNA) RAPD analysis.The results showed that difference was between not only the cms line and its maintainer,but also cms line and its F 1 hybrid.Primer OPJ-08 generated a polymorphic product of 800 bp in sterile line,and primer OPK-10 amplified a specific fragment of 900 bp in F 1 hybrid.The two fragments were then cloned into plasmid vector and converted to RFLP probes.Southern hybridization patterns revealed polymorphism between sterile line and F 1 hybrid using OPJ-08 800 fragment as probe,and between sterile line and maintainer line using OPK-10 900 fragment as probe.We infered that the two mtDNA fragments may be associated with cms in rice.