HPLC determination of paeoniflorin in Danggui Shaoyao granules

To establish a method for determining the concentration of paeoniflorin in Danggui Shaoyao granules.Methods:The sample was extracted with methanol on 60℃ water environment and then purified by alu- minum oxide column.HPLC was used for the determination of paeoniflorin in Danggui Shaoyao granules.The chrom- atographic procedure was carried out using Nucleosil-C_(18)(200 mm×4. 6 mm,7μm)as an analytical column and a mixture of 6.5 volume of isopropanol,93.3 volume of water,and 0.2 volume of acetic acid as mobile phase at a flow rate of 1.5 mL·min~(-1).The detection wavelength was set at 230 nm.Results:The peaks of paeoniflorin appeared on about 15 minutes and the resolutions of them were more than 1.5.Their linear ranges were between 0.1 and 1.2 g(r=0.9999),with the average recoveries of 96.6%(n=9).Conclusion:This method is sensitive,quick and ac- curate for the measurement of paeoniflorin in Danggui Shaoyao granules.