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RP-HPLC method for the content determination of paeoniflorin in Danggui Shaoyao San

Xiaohua Pang

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Abstract

Objective:To establish the method for the content determination of paeoniflorin in Danggui Shaoyao San. Methods: External standard method was conducted with the column of C18(150 mm×4.6 mm,5 μm), the mobile phase was methanol-0.5% acetic acid(30∶70), the flow rate was 1.0 ml/min, and the detective wavelength was set at 230 nm. Results: There was a good linearity in the range of 20~100 μg/ml(r=0.999 6);the average recovery was (99.9±1.6)% with RSD of 1.14%.Conclusion: The assaying method of RP-HPLC is convenient, accurate, and reliable with good precision, and can be used in the quality control of Danggui Shaoyao San.

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What this paper is about

Objective:To establish the method for the content determination of paeoniflorin in Danggui Shaoyao San. Methods: External standard method was conducted with the column of C18(150 mm×4.6 mm,5 μm), the mobile phase was methanol-0.5% acetic acid(30∶70), the flow rate was 1.0 ml/min, and the detective wavelength was set at 230 nm. Results: There was a good linearity in the range of 20~100 μg/ml(r=0.999 6);the average recovery was (99.9±1.6)% with RSD of 1.14%.Conclusion: The assaying method of RP-HPLC is convenient, accurate, and reliable with good precision, and can be used in the quality control of Danggui Shaoyao San.

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Available abstract

Objective:To establish the method for the content determination of paeoniflorin in Danggui Shaoyao San. Methods: External standard method was conducted with the column of C18(150 mm×4.6 mm,5 μm), the mobile phase was methanol-0.5% acetic acid(30∶70), the flow rate was 1.0 ml/min, and the detective wavelength was set at 230 nm. Results: There was a good linearity in the range of 20~100 μg/ml(r=0.999 6);the average recovery was (99.9±1.6)% with RSD of 1.14%.Conclusion: The assaying method of RP-HPLC is convenient, accurate, and reliable with good precision, and can be used in the quality control of Danggui Shaoyao San.

Key concepts: Paeoniflorin, Medicine, Content determination, Chromatography, High-performance liquid chromatography, Traditional medicine, Acetic acid, Biochemistry

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RP-HPLC method for the content determination of paeoniflorin in Danggui Shaoyao San — Research Paper | ScholarLens