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2005•The Chinese Journal of Modern Applied PharmacyRequires access

Determination of paeoniflorin in Ejiao Danggui mixture

Hang Qing, Xu Xiang, Weihong Ge

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Abstract

OBJECTIVE To develop an effective quality control method for Ejiao Danggui mixture.METHODS Paeoniflorin in Ejiao Danggui mixture was determined by RP-HPLC,using Zorbax XDB-C_(18)(3.9×150mm,5μm)column,methanol-water-0.1%phosphoric acid(35∶65∶0.1) as mobile phase and UV detector at 230 nm.The flow rate was 0.8 mL·min~(-1).RESULTS The linear range of paeoniflorin was 3.395～33.95μg·mL~(-1),the average recovery of paeoniflorin was 96.1% with RSD 0.88%.CONCLUSION The method is simple,rapid,accurate and repreatable.It can be used for determination of paeoniflorin in Ejiao Danggui mixture.

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What this paper is about

OBJECTIVE To develop an effective quality control method for Ejiao Danggui mixture.METHODS Paeoniflorin in Ejiao Danggui mixture was determined by RP-HPLC,using Zorbax XDB-C_(18)(3.9×150mm,5μm)column,methanol-water-0.1%phosphoric acid(35∶65∶0.1) as mobile phase and UV detector at 230 nm.The flow rate was 0.8 mL·min~(-1).RESULTS The linear range of paeoniflorin was 3.395～33.95μg·mL~(-1),the average recovery of paeoniflorin was 96.1% with RSD 0.88%.CONCLUSION The method is simple,rapid,accurate and repreatable.It can be used for determination of paeoniflorin in Ejiao Danggui mixture.

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Available abstract

OBJECTIVE To develop an effective quality control method for Ejiao Danggui mixture.METHODS Paeoniflorin in Ejiao Danggui mixture was determined by RP-HPLC,using Zorbax XDB-C_(18)(3.9×150mm,5μm)column,methanol-water-0.1%phosphoric acid(35∶65∶0.1) as mobile phase and UV detector at 230 nm.The flow rate was 0.8 mL·min~(-1).RESULTS The linear range of paeoniflorin was 3.395～33.95μg·mL~(-1),the average recovery of paeoniflorin was 96.1% with RSD 0.88%.CONCLUSION The method is simple,rapid,accurate and repreatable.It can be used for determination of paeoniflorin in Ejiao Danggui mixture.

Key concepts: Paeoniflorin, Chromatography, Phosphoric acid, High-performance liquid chromatography, Chemistry, Organic chemistry

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