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2007•Chinese Journal of Modern Drug ApplicationRequires access

Determination of paeoniflorin in Shaoyao Pil by HPLC

Ruimin Li

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Abstract

Objective To establish a HPLC method for the determination of paeoniflorin in Shaoyao Pil.Methods The samples was analysed on the Inertsil ODS-3 C18 column (250 mm×4.6 mm,5 μm) with the column temperature at 40 ℃.The mobile phase was methanol-1%phosphoric acid solution(35:36).The detector wave length was set at 230 mm.Results The good linear relationship was obtained in the range of 4.92~49.2 μg/ml of paeoniflorin(r =0.999 ),and the average recovery was 98.64%.Conclusion The method was quick, simple and accurate.It is suitable for quality control in Shaoyao Pil.

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What this paper is about

Objective To establish a HPLC method for the determination of paeoniflorin in Shaoyao Pil.Methods The samples was analysed on the Inertsil ODS-3 C18 column (250 mm×4.6 mm,5 μm) with the column temperature at 40 ℃.The mobile phase was methanol-1%phosphoric acid solution(35:36).The detector wave length was set at 230 mm.Results The good linear relationship was obtained in the range of 4.92~49.2 μg/ml of paeoniflorin(r =0.999 ),and the average recovery was 98.64%.Conclusion The method was quick, simple and accurate.It is suitable for quality control in Shaoyao Pil.

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Available abstract

Objective To establish a HPLC method for the determination of paeoniflorin in Shaoyao Pil.Methods The samples was analysed on the Inertsil ODS-3 C18 column (250 mm×4.6 mm,5 μm) with the column temperature at 40 ℃.The mobile phase was methanol-1%phosphoric acid solution(35:36).The detector wave length was set at 230 mm.Results The good linear relationship was obtained in the range of 4.92~49.2 μg/ml of paeoniflorin(r =0.999 ),and the average recovery was 98.64%.Conclusion The method was quick, simple and accurate.It is suitable for quality control in Shaoyao Pil.

Key concepts: Paeoniflorin, Chromatography, Chemistry, Phosphoric acid, High-performance liquid chromatography, Content determination, Organic chemistry

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