Synthesis and Antifungal Activities in Vitro of 2,3,3a,4-Tetrahydrothiochromeno[4,3-c]pyrazole Derivatives

OBJECTIVE To design and synthesize 2,3,3a,4-tetrahydrothiochromeno[4,3-c]pyrazole derivatives and measure their antifungal activity in vitro.METHODS The intermediate of 3-benzylidene-1-thiochromanones was synthesized from the substituted benzenethiols.secondly,the target compounds were obtained by the reactions of hydrazine with 3-benzylidene-1-thiochromanones in acetic acid.The antifungal activity of the target compounds in vitro was measured by consecutive double dilution.RESULTS Eight new 2,3,3a,4-tetrahydrothiochromeno[4,3-c]pyrazole derivatives were synthesized,and then their structures were identified by 1H-NMR,MS and elemental analysis.The result of antifungal activity indicated that the target compounds had good antifungal effects on most tested fungi.CONCLUSION 2,3,3a,4-tetrahydrothiochromeno-[4,3-c]pyrazole derivatives show antifungal activity in vitro,which required further research in the future.