Synthesis of 2,3,3a,4-tetrahydrothiochromenopyrazoles derivatives and their antifungal activity in vitro

Aim To synthesize 2,3,3a,4-tetrahydrothiochromeno[4,3-c]pyrazoles derivatives and measure their antifungal activity.Methods Substituted benzenethiols were used as starting materials.Firstly they were transformed into 3-arylidene-1-thiochromanones.Secondly the target compounds were prepared by the condensation of hydrazine with the intermediates in hot acetic acid.And their antifungal activity was tested by consecutive double dilution for E.floccosum,M.gypseum,T.viride,and T.tonsurans.Results Twelve new 2,3,3a,4-tetrahydrothiochromenopyrazoles derivatives were synthesized,and their structures were conformed by 1H-NMR,MS and element analysis.The result of antifungal experiment indicated that the target compounds had good antifungal effect on most tested fungi in vitro.Conclusion 2,3,3a,4-tetrahydrothiochromenopyrazoles derivatives showed certain antifungal activity in vitro.