The serum E-selectin level and the G98T and S128R polymorphisms of E-selectin in patients with acute myocardial infarction

Objective To study the relationship between serum levels of E-selectin or the G98T polymorphism in the exon 2 and the S128R polymorphism in the exon 4 of E-selectin gene and acute myocardial infarction in Chinese Han peoples. Methods The genotypes of E-selectin were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methods in 185 acute myocardial infarction patients and 190 healthy controls, and the serum level of E-selectin was determined by enzyme-linked immunosorbent assay ( ELISA). Results Acute myocardial infarction group showed significantly higher serum levels of E-selectin than that of control group ( P 0. 01) , There was significant difference in frequencies of allele and genotype in S128R polymorphism between acute myocardial infarction and control groups respectively. The relative risk suffered from acute myocardial infarction of SS genotype was 2. 226 times of the SR genotype ( OR = 2. 226,95% CI: 1. 106 - 4. 481) , the serum E-selectin level was significantly higher among carriers of SR genotype as compared with non-carriers(GG genotype) (41. 93 ± 8.94 μg/L vs (35.75 ±6.83) μg/L, P 0. 05). There was no significant difference in genotype distribution for the E-selectin gene G98T genotype between acute myocardial infarction and control groups (P0.05). Conclusion E-selectin S128R polymorphism was associated with acute myocardial infarction, its polymorphism may affect the serum E-selectin level, and R allele may be a risk factor for acute myocardial infarction. The polymorphism of E-selectin gene G98T maybe play a minimum role in the pathogenesis of acute myocardial infarction.