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2013•Unpublished venueRequires access

Simultaneous Determination of Three Kinds Chemical Contents in Flos lonicerae by HPLC

Wang Jian-wei

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Abstract

Objective:To establish a high performance liquid chromatographic method for simultaneous determination of the contents of Chlorogenic acid,Rutin and Luteolin-7-O-glucoside in Flos lonicerae.Methods:HPLC was applied with the chromatographic condition as follows:the chromatographic column was Agilent Zorbax SB-C18(4.6mm×250mm,5 μm)at 30 ℃;acetonitrile-0.1%H3PO4 as the mobile phrase,gradient elution;flow rate was 1.0 mL·min-1,the detection wavelength was 355 nm.Results:Linearity relationships were 12.21～203.5 μg·mL-1(r=0.9992),2.653～132.6 μg·mL-1(r=0.9999),2.794～139.7 μg·mL-1(r=0.9995),respectively;the average recoveries were 98.60%(RSD=158%),98.99%(RSD=1.65%),99.19%(RSD=1.26%).Conclusion:The results showed that the method is simple,accurate and repeatable and it is suitable for simultaneous determining the contents of Rutin,Hesperidin and Arctiin in Flos lonicerae.

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What this paper is about

Objective:To establish a high performance liquid chromatographic method for simultaneous determination of the contents of Chlorogenic acid,Rutin and Luteolin-7-O-glucoside in Flos lonicerae.Methods:HPLC was applied with the chromatographic condition as follows:the chromatographic column was Agilent Zorbax SB-C18(4.6mm×250mm,5 μm)at 30 ℃;acetonitrile-0.1%H3PO4 as the mobile phrase,gradient elution;flow rate was 1.0 mL·min-1,the detection wavelength was 355 nm.Results:Linearity relationships were 12.21～203.5 μg·mL-1(r=0.9992),2.653～132.6 μg·mL-1(r=0.9999),2.794～139.7 μg·mL-1(r=0.9995),respectively;the average recoveries were 98.60%(RSD=158%),98.99%(RSD=1.65%),99.19%(RSD=1.26%).Conclusion:The results showed that the method is simple,accurate and repeatable and it is suitable for simultaneous determining the contents of Rutin,Hesperidin and Arctiin in Flos lonicerae.

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Available abstract

Objective:To establish a high performance liquid chromatographic method for simultaneous determination of the contents of Chlorogenic acid,Rutin and Luteolin-7-O-glucoside in Flos lonicerae.Methods:HPLC was applied with the chromatographic condition as follows:the chromatographic column was Agilent Zorbax SB-C18(4.6mm×250mm,5 μm)at 30 ℃;acetonitrile-0.1%H3PO4 as the mobile phrase,gradient elution;flow rate was 1.0 mL·min-1,the detection wavelength was 355 nm.Results:Linearity relationships were 12.21～203.5 μg·mL-1(r=0.9992),2.653～132.6 μg·mL-1(r=0.9999),2.794～139.7 μg·mL-1(r=0.9995),respectively;the average recoveries were 98.60%(RSD=158%),98.99%(RSD=1.65%),99.19%(RSD=1.26%).Conclusion:The results showed that the method is simple,accurate and repeatable and it is suitable for simultaneous determining the contents of Rutin,Hesperidin and Arctiin in Flos lonicerae.

Key concepts: Rutin, Chromatography, Flos, Chemistry, Hesperidin, Chlorogenic acid, High-performance liquid chromatography, Gradient elution

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