Determination of the Chlorogenic Acid in Flos Lonicerae with HPLC

The high performance liquid chromatographic method was developed for the determination of chlorogenic acid in Flos Lonicerae.The chromatographic separation was carried out in a Nucleosil C18 column(4.6×250 mm,5 μm) with a mobile phase consisting of methanol-0.4 %H3PO4(25∶75,V/V) mixture.The detection wavelength of photodiode array detector was set at 327 nm and the column temperature was set at 25 ℃.The calibration curve was linear in the range of 0.64～6.40 μg for chlorogenic acid(r=0.999 6).The average recovery of chlorogenic acid was 99.3 % with a relative standard deviation(RSD)of 0.8 %.The method was simple,reliable and suitable for quality evaluation and control of Flos Lonicerae.