ScholarLens Back to search
2007•Central South PharmacyRequires access

HPLC determination of chlorogenic acid in Flos Lonicerae

XU Kang-ping

Open publisher page 0 citations

Abstract

Objective To establish a method for the determination of chlorogenic acid in Flos Lonicerae.Methods HPLC method was adopted.The Hypersil BDS C18(4.6 mm×200 mm,5 μm) was used.The mobile phase consisted of MeOH-H2O(The water was adjusted with phosphoric acid to pH 3.0)=18∶82.The flow rate was 1.0 mL·min-1.The detective wavelength was 323 nm.Results The calibration curves of chlorogenic acid was in good linearity over 0.418～16.704 μg(r=0.999 9).The average recovery was 97.67%,and RSD was 1.25%(n=6).Conclusion The method is simple,sensitive,accurate and reproducible,and can be used to control the quality of Flos Lonicerae.

About this research paper

What this paper is about

Objective To establish a method for the determination of chlorogenic acid in Flos Lonicerae.Methods HPLC method was adopted.The Hypersil BDS C18(4.6 mm×200 mm,5 μm) was used.The mobile phase consisted of MeOH-H2O(The water was adjusted with phosphoric acid to pH 3.0)=18∶82.The flow rate was 1.0 mL·min-1.The detective wavelength was 323 nm.Results The calibration curves of chlorogenic acid was in good linearity over 0.418～16.704 μg(r=0.999 9).The average recovery was 97.67%,and RSD was 1.25%(n=6).Conclusion The method is simple,sensitive,accurate and reproducible,and can be used to control the quality of Flos Lonicerae.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective To establish a method for the determination of chlorogenic acid in Flos Lonicerae.Methods HPLC method was adopted.The Hypersil BDS C18(4.6 mm×200 mm,5 μm) was used.The mobile phase consisted of MeOH-H2O(The water was adjusted with phosphoric acid to pH 3.0)=18∶82.The flow rate was 1.0 mL·min-1.The detective wavelength was 323 nm.Results The calibration curves of chlorogenic acid was in good linearity over 0.418～16.704 μg(r=0.999 9).The average recovery was 97.67%,and RSD was 1.25%(n=6).Conclusion The method is simple,sensitive,accurate and reproducible,and can be used to control the quality of Flos Lonicerae.

Key concepts: Chlorogenic acid, Flos, Phosphoric acid, Chromatography, Chemistry, Calibration curve, High-performance liquid chromatography, Rutin

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
HPLC determination of chlorogenic acid in Flos Lonicerae — Research Paper | ScholarLens