EXPERIMENTAL OBSERVATION ON GERMICIDAL EFFICACY AND TOXICITY OF A HAND-DISINFECTION SOLUTION

Jin-Kang hand-disinfection solution is a compound disinfection solution with polyhexamethylene guanidine hydrochloride as the main ingredient.In order to observe its germicidal efficacy and safety for use, suspension quantitative germicidal test and animal toxicity test was used to carry out laboratory study.Results: The reduction factor(log_(10)) of Staphylococcus aureus and Pseudomonas aeruginosa in suspension exposed to the hand-disinfection solution containing polyhexamethylene guanidine hydrochloride 260 mg/L for 1.5 min attained over 5.00 and that of Escherichia coli exposed for 3 min also attained over 5.00.The reduction factor(log_(10)) of Candida albicans exposed to the disinfection solution containing polyhexamethylene guanidine hydrochloride 520 mg/L for 1.5 min was higher than 4.00.Wiping disinfection with the stock solution of this disinfection solution for 3 min reduced the natural bacteria on hands of the test subjects by 10~(2.4) in average.The mouse oral LD_(50) of the hand-disinfection solution was 5000 mg/kg·bw,indicating that it was practically non-toxic.The average skin irritation score of its stock solution was 0 and its mouse accumulation coefficient K was 5.Conclusions: The hand-disinfection solution has good efficacy in killing vegetative forms of bacteria and yeasts.It belongs to nontoxic substances,has no irritating effect on skin and weak cumulative toxicity and does not cause micronucleus formation.