EXPERIMENTAL STUDY ON GERMICIDAL EFFICACY AND TOXICITY OF POLYHEXAMETHYLENE BIGUANIDINE DISINFECTION SOLUTION

Objective To observe the germicidal efficacy and toxicity of polyhexamethylene biguanidine hydrochloride disinfection solution.Methods Suspension quantitative germicidal test and animal toxicity test were used to carry out laboratory observation on its germicidal efficacy and toxicity.Results The average killing rates of Escherichia coli in suspension exposed to the disinfectant solution containing polyhexamethylene biguanidine hydrochloride 1800 mg/L for 5 min was 99.95%,that of Staphylococcus aureus exposed to the solution containing polyhexamethylene biguanidine hydrochloride 2400 mg/L for 5 min was 99.91% and that of Candida albicans exposed to the solution containing polyhexamethylene biguanidine hydrochloride 3200 mg/L for 10 min was 99.95%.More than 25%(v/v) calf serum in the bacterial suspension slightly influenced its efficacy in killing Escherichia coli.The mouse acute oral LD50 was above 5000 mg/kg.The rabbit skin irritation test index of the solution containing polyhexamethylene biguanidine hydrochloride 5200 mg/L was 0.32,which belongs to mild irritation.Conclusions Polyhexamethylene biguanidine disinfection solution kills effectively the vegetative forms of bacteria and Candida albicans.This disinfectant belongs to practically non-toxic class and was slightly irritative to skin.