Immunization effect of two kinds of domestic adjuvant-free rabies vaccine

Objective The present study was designed to evaluate CTN-1 Rabies vaccine purified by vero cell rabies vaccine (freeze-dried) and aG strain Rabies vaccine prepared by Primary Hamster Kidney Cells (liquid), both of which were Adjuvant-free, for their immune effects.Methods Patients with WHO category II exposure were divided into 2 groups,35 Patients in group 1 , immunized with CTN-1 (Vero cell)Rabies Vaccine manufactured by Wuhan Institute of biological products, the other 46 in group 2 were inoculated by aG strain Rabies vaccine(PHKC) produced in Henan Puxin Biotech Co., Ltd.The post-exposure immunizations were given on days 0, 3rd, 7th,14th and 28th to these two groups. observation on adverse reaction was carried out after immunization, Serum samples were collected and rabies virus neutralizing antibody (RVNA) titers were tested by rapid fluorescent focus inhibition test (RFFIT) at 7 and 14 days after the primary immunization. Results Seroconversion rate in group 1 and 2 were 58.52% and 65.22% respectively at 7 days after the first immunization, there was no significant difference between the two groups as far as immune effects were concerned, and both groups had a positive seroconversion rate of 100% on the 14th day; No abnormal reaction was found in CTN-1 groups,only one person in aG group had a 4×4 sized red swelling after the second inoculation.Conclusion These two kinds of domestically produced adjuvant-free rabies vaccine can both elicit protection antibodies efficiently , and the CTN-1 rabies vaccine (Vero cell, freeze-dried) provided more effective and specific immune protection than aG strain vaccine(PHKC, liquid).