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Content determination of costunolide in Liuwei muxiang capsule by RP-HPLC

Zhun Li

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Abstract

Objective To establish the content determination method of costunolide in Liuwei muxiang capsule(traditional Chinese medicines).Methods A reversed-phase high performance liquid chromatographic method was used.The measurement conditions were as follows:kromasil C18column(4.6 mm×200 mm,5 μm);detection wavelength:225 nm;mobile phase:methanol-water(V∶V=65∶35);flow rate :1.0 mL·min-1.Results The linear ranges of costunolide was 22.5-180.0mg· L-1(r=0.999 9).The mean recovery was 98.3%,RSD= 0.7%(n=9).Conclusions The method is suitable for content determination of costunolide in Liuwei muxiang capsule.

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What this paper is about

Objective To establish the content determination method of costunolide in Liuwei muxiang capsule(traditional Chinese medicines).Methods A reversed-phase high performance liquid chromatographic method was used.The measurement conditions were as follows:kromasil C18column(4.6 mm×200 mm,5 μm);detection wavelength:225 nm;mobile phase:methanol-water(V∶V=65∶35);flow rate :1.0 mL·min-1.Results The linear ranges of costunolide was 22.5-180.0mg· L-1(r=0.999 9).The mean recovery was 98.3%,RSD= 0.7%(n=9).Conclusions The method is suitable for content determination of costunolide in Liuwei muxiang capsule.

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Available abstract

Objective To establish the content determination method of costunolide in Liuwei muxiang capsule(traditional Chinese medicines).Methods A reversed-phase high performance liquid chromatographic method was used.The measurement conditions were as follows:kromasil C18column(4.6 mm×200 mm,5 μm);detection wavelength:225 nm;mobile phase:methanol-water(V∶V=65∶35);flow rate :1.0 mL·min-1.Results The linear ranges of costunolide was 22.5-180.0mg· L-1(r=0.999 9).The mean recovery was 98.3%,RSD= 0.7%(n=9).Conclusions The method is suitable for content determination of costunolide in Liuwei muxiang capsule.

Key concepts: Capsule, Chromatography, High-performance liquid chromatography, Chemistry, Content determination, Biology, Botany

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