2011•Journal of Inner Mongolia University for the NationalitiesRequires access

Determination the Content of Costunolide in Mongolian Medicine Gagula-4 Decoction by RP-HPLC

Dongmei Bao

Abstract

Objective:To establish a content determination method of costunolide in Mongolian medicine Gagula-4 decoction.Methods:The reversed phase high performance liquid chromatography(RP-HPLC) separation was performed on Hypersil ODS-C18 column(4.6mm×250mm,5μm),the mobile phase wasmethanol-water(65:35,) the flow rate was 0.8ml·min-1,the detection wavelength was 225nm,the columntemperature was 25℃.Results:Costunolide showed a good linear relationship(r=0.995)in the range of 0.20~ 1.00μg,the average recovery rate was 99.73%,RSD=1.545%.Conclusion:The method is simple and accurate,and has a high resolution,so it can be served as the quality control of costunolide in Mongolian medicine Gagula-4 decoction.

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Key concepts: Decoction, Chromatography, High-performance liquid chromatography, Content determination, Recovery rate, Linear relationship, Chemistry, High resolution