Determination of costunolide in Muxiang Shunqi pills by HPLC

OBJECTIVE To determine the content of costunolide in the Muxiang Shunqi pills of different batches.METHODS HPLC analysis was adopted with the column of a Kromasil C18(250 mm×4.6 mm,5 μm) at 30 ℃ as column temperature.The mobile phase was methanol-water(60:40) with detection wavelength of 225 nm and the flow rate was 1.0 mL·min-1.The injection volume was 10 μL.RESULTS Costunolide showed a good linear relationship between area integration value and sampling amount at a range of 1.033-20.67 μg·mL-1(r=0.9999),with the average recovery of 98.95% and RSD=1.2%(n=9).The mean content of costunolide in 5 batches of Muxiang Shunqi pills was 0.08%.CONCLUSION The method is simple and stable,which can be used the quality evaluation of Muxiang Shunqi pills.