2013•Huaxi yaoxue zazhiRequires access

Determination of costunolide in Fufang Muxiang Xiaobaijian tablet by HPLC

Jun Liu

Abstract

OBJECTIVE To determination the content of costunolide in the Fufang Muxiang Xiaobaijian tablet of different batches.METHODS The column was Kromasil C 18(250 mm × 4.6 mm,5 μm),and the mobile phase was methanol-water(70∶30) by HPLC.The detection wavelength was 225 nm,with a flow rate of 1.0 mL.min-1,a column temperature of 25 ℃,and a injection volume of 10 μL.RESULTS In the range of 10.78-107.8 μg.mL-1,costunolide showed a good linear relationship(r = 0.9999,n = 6),with average recovery of 99.86%,RSD = 1.4%(n = 9).The content of costunolide in Fufang Muxiang Xiaobaijian tablet from five batches was 0.08%.CONCLUSION The method is simple,stable.The content of costunolide can be used as one of the bases for the quality evaluation of Fufang Muxiang Xiaobaijian tablet.

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Key concepts: Chemistry, Chromatography, High-performance liquid chromatography, Content determination, Linear relationship, Mathematics, Statistics