2009•Central South PharmacyRequires access

Content determination of paeoniflorin and ferulic acid in Liuwei Buxue granules by HPLC

Lin Zhou

Abstract

Objective To establish a method for the content determination of paeoniflorin and ferulic acid in Liuwei Buxue granules by HPLC.Methods Ultimate XB-C18 column(250 mm×4.6 mm,5 μm) was used.The mobile phase was acetonitrile-0.1% phosphoric acid solution(15∶85),the detection wavelenghth was 230 nm for paeoniflorin and 323 nm for ferulic acid,the flow rate was 1.0 mL·min-1,and column temperature was 25℃.Results The linear range of paeoniflorin and ferulic acid was 0.108～0.864 μg(r=0.999 6,n=6)and 0.044 0～0.352 μg(r=0.999 8,n=6).The average recovery of paeoniflorin and ferulic acid was 97.77% and 96.78%,RSD(n=9) was 1.33% and 1.08%.Conclusion The method may be used for the content determination of paeoniflorin and ferulic acid in Liuwei Buxue granules.

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Available abstract

Key concepts: Paeoniflorin, Ferulic acid, Chemistry, Chromatography, Phosphoric acid, High-performance liquid chromatography, Content determination, Organic chemistry