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Determination of ferulic acid and paeoniflorin in Xiaoyao Wan by RP-HPLC

Xin Wan

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Abstract

OBJECTIVE To develop a method for the determination of ferulic acid and paeoniflorin in Xiaoyao Wan by RP-HPLC.METHODS The chromatographic conditions included Shimpack CLC ODS column and the mobile phase consisting of a mixture of acetonitrile-0.1% phosphoric acid (15∶85).The detection wavelength was at 230 nm.RESULTS The calibration curve was linear over the concentration range of 0.02～0.12 mg for ferulic acid and 0.02～0.08 mg for paeoniflorin respectively.The correlation coefficient was r =0.997 8 and r = 0.999 9 .The average recovery of ferulic acid was 97.61% and that of paeoniflorin was 100.07%.The precision of within-day RSD was 0.98% and 0.72% respectively.CONCLUSION The determination of ferulic acid and paeoniflorin was found to be simple and accurate.

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OBJECTIVE To develop a method for the determination of ferulic acid and paeoniflorin in Xiaoyao Wan by RP-HPLC.METHODS The chromatographic conditions included Shimpack CLC ODS column and the mobile phase consisting of a mixture of acetonitrile-0.1% phosphoric acid (15∶85).The detection wavelength was at 230 nm.RESULTS The calibration curve was linear over the concentration range of 0.02～0.12 mg for ferulic acid and 0.02～0.08 mg for paeoniflorin respectively.The correlation coefficient was r =0.997 8 and r = 0.999 9 .The average recovery of ferulic acid was 97.61% and that of paeoniflorin was 100.07%.The precision of within-day RSD was 0.98% and 0.72% respectively.CONCLUSION The determination of ferulic acid and paeoniflorin was found to be simple and accurate.

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Available abstract

OBJECTIVE To develop a method for the determination of ferulic acid and paeoniflorin in Xiaoyao Wan by RP-HPLC.METHODS The chromatographic conditions included Shimpack CLC ODS column and the mobile phase consisting of a mixture of acetonitrile-0.1% phosphoric acid (15∶85).The detection wavelength was at 230 nm.RESULTS The calibration curve was linear over the concentration range of 0.02～0.12 mg for ferulic acid and 0.02～0.08 mg for paeoniflorin respectively.The correlation coefficient was r =0.997 8 and r = 0.999 9 .The average recovery of ferulic acid was 97.61% and that of paeoniflorin was 100.07%.The precision of within-day RSD was 0.98% and 0.72% respectively.CONCLUSION The determination of ferulic acid and paeoniflorin was found to be simple and accurate.

Key concepts: Paeoniflorin, Ferulic acid, Chromatography, Phosphoric acid, High-performance liquid chromatography, Chemistry, Calibration curve, Acetonitrile

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