Simultaneous determination of paeoniflorin,ferulic acid and rutin in Xinning tablets by RP-HPLC

Objective To develop an RP-HPLC method for simultaneous determination of paeoniflorin,ferulic acid and rutin in Xinning(traditional Chinese medicines)tablets.Methods The analysis was performed on a Diamonsil C18 column(200 mm×4.6 mm,5 μm) with a mobile phase of methanol-acetonitrile-1% acetic acid(V∶V∶V=25∶3∶70).The flow rate was 1.0 mL·min-1 and column temperature was 35 ℃.The detection wavelength was set at 230 nm.Results The calibration curve was linear within the range of 51.02-510.20 mg·L-1(r=0.999 9) for paeoniflorin,1.08-10.80 mg·L-1(r=0.999 9) for ferulic acid and 19.04-190.40 mg·L-1(r=0.999 6)for rutin,respectively.The recoveries(n=9) of paeoniflorin,ferulic acid and rutin were 100.5%(RSD=1.0%),100.4%(RSD=0.8%),and 100.4%(RSD=0.9%),respectively.Conclusions The method is simple,accurate and selective.It can be applied to determine paeoniflorin,ferulic acid and rutin in Xinning tablets.