Simultaneous Determination of Paeoniflorin and Ferulic Acid in Buyanghuanwu Decoction by RP-HPLC

Objective To establish a high performance liquid chromatography(HPLC) for simultaneous determination of paeoniflorin and ferulic acid in buyanghuanwu decoction. Methods The condition of determination was optimized.Waters Symmetry C_(18) column(4.6 mm×150 mm,5 μm) was used at 30 ℃ and mobile phase was 1%acetic acid-methanol(70∶30)(pH 3.15)with a flow rate of 1.0 mL·min~(-1).The detection wavelength for paeoniflorin and ferulic acid were 230 nm and 320 nm,respectively. Results The linearity range of paeoniflorin and ferulic were 4-24 μg·mL~(-1) and 0.4-2.0 μg·mL~(-1),respectively,the correlation coefficient were 0.999 9 and 0.999 8,respectively,the average recoveries(n=3) were 101.72%(RSD=0.27%)and 104.72%(RSD=0.46%). Conclusion This is a simple and accurate detection method used for the quality control of that prescription.