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Study on Tissue Culture and Rapid Propagation of Thornless Red Raspberry Idaeus

Lei Chen

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Abstract

Using stem segments with buds and tips as explants,tube rapid propagation of Red Raspberry was studied.The results indicated that the appropriate media for respective culture stages were: for germination of auxiliary buds: 1/2 MS+6-BA(1.0 mg/L);for differentiation and multiplication: MS+6-BA 1.0 mg/L+NAA 0.1 mg/L,the multiple for multiplication was up to 7;for rooting: 1/2MS+IBA(1.0 mg/L+)active carbon 1.0 g/L and the rate of rooting was 93.33%.After 20-day acclimatization,the rooted plantlets were transplanted to substrata in two successive steps,the survival rates of which were 98% and 100% respectively.

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What this paper is about

Using stem segments with buds and tips as explants,tube rapid propagation of Red Raspberry was studied.The results indicated that the appropriate media for respective culture stages were: for germination of auxiliary buds: 1/2 MS+6-BA(1.0 mg/L);for differentiation and multiplication: MS+6-BA 1.0 mg/L+NAA 0.1 mg/L,the multiple for multiplication was up to 7;for rooting: 1/2MS+IBA(1.0 mg/L+)active carbon 1.0 g/L and the rate of rooting was 93.33%.After 20-day acclimatization,the rooted plantlets were transplanted to substrata in two successive steps,the survival rates of which were 98% and 100% respectively.

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Available abstract

Using stem segments with buds and tips as explants,tube rapid propagation of Red Raspberry was studied.The results indicated that the appropriate media for respective culture stages were: for germination of auxiliary buds: 1/2 MS+6-BA(1.0 mg/L);for differentiation and multiplication: MS+6-BA 1.0 mg/L+NAA 0.1 mg/L,the multiple for multiplication was up to 7;for rooting: 1/2MS+IBA(1.0 mg/L+)active carbon 1.0 g/L and the rate of rooting was 93.33%.After 20-day acclimatization,the rooted plantlets were transplanted to substrata in two successive steps,the survival rates of which were 98% and 100% respectively.

Key concepts: Blowing a raspberry, Explant culture, Acclimatization, Germination, Tissue culture, Biology, Botany, Horticulture

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Study on Tissue Culture and Rapid Propagation of Thornless Red Raspberry Idaeus — Research Paper | ScholarLens