Study on the Tissue Culture Technique in Raspberry

The objective of this research was to study the tissue culture system of raspberry through explants of raspberry stem segment with buds,especially on optimum formula of culture medium and culture procedure at each phase.The results showed that the optional time of cutting explant was in early May,that the best explant was semi-lignified stem segment with buds,disinfected by 75% alcohol for 0.5 min and 2% NaClO for 15 min,that the optimal differentiation and rooting medium were MS+6-BA 1.0 mg/L+NAA 0.02 mg/L+GA3 6 mg/L and 1/2MS+NAA 2.0 mg/L+IBA 1.0 mg/L+activated carbon 0.05% respectively,that the cultured plantlets at the highest survival rate was transplanted in organic fertilizer,earth and vermiculite(1∶1∶1).