Study on Microspore Embryogenesis,Regeneration and Propagationin vitroon Ornamental Kale(Brassica oleraceavar.acephala)

Using twenty materials in ornamental kale(Brassica oleracea L.var.acephala DC.)as material,using microspore culture,influence factors of embryogenesis,plant regeneration and the subculture of multiple shoots were studied systematically.The results showed that the genotype was one of the most important factors to embryogenesis.Fifteen genotypes produced embryos with a seventy-five percent induction ratio.The optimal sucrose concentration was also associated with genotypes.In different genotypes,the best ratio of 6-BA and NAA was 1∶1or 2∶1.Compared with B5 medium,MS was better in plant regeneration.The best subculture duration shoots was 15 days with an average propagation coefficient of 10.3.