Establishment and Application of the System for Isolated Microspore Culture in Kale (Brassica oleracea L.var.acephala DC.)

Isolated microspore culture was carried out by ten hybrids of kale (Brassica oleracea L. var. acephala DC.). The frequency of embryogenesis, embryos germinated plantlets, ploidy identification of microspores-derived plantlets and chromosome doubling techniques for haploid plantlets were studied. The results showed that the buds in flourishing florescence were the best for the purpose of microspore culture. The sucrose content of 13% was optimal in medium. MS+6-BA 2 mg·L-1+ NAA 0.1 mg·L-1 was optimal medium for subculture and the average propagation quotiet reached 5.06. The survival rate of microspore-derived plantlets reached 74.6%. The DH plants rate of spontaneous was in the range of 23.33%-37.50%. The treatment of 70 mg·L-1 colchicine for 9-11 d was optimal for haploid seedlings and led to 54.55% doubled haploid plantlets.