Highly efficient microspore embryogenesis and plant regeneration in early maturing cauliflowers(Brassica oleracea var. botrytis)

Seven early-maturing hybrids and one medium-maturing hybrid of cauliflowers(Brassica oleracea var.botrytis) were applied to isolated microspore culture.The results showed that the microspore embryogenesis in early-maturing cauliflowers was induced in almost donor plants in spite of showing genotype-dependency from the lowest nothing to the highest 112 embryos per flower bud.The highest average embryo yield was 'Xiaxue 40',which produced 45.6 embryos per bud,while did 'Zhaohua 45' null.The germinated rate for embryos was normally around 30%,while two genotypes was over 80% but one got nothing.The great variant in growth stage and fertility was observed in regenerated plants.