Isolated Microspore Culture and Ploidy Variation of Regenerated Plants in Ornamental Kale

Five F1 hybrids of ornamental kale( Brassica oleracea L. var. acephala) were used in isolated microspore culture in order to obtain diverse ornamental kale DH lines and lay the foundation of materials for breeding excellent varieties of ornamental kale. The results showed that the genotype affected the frequency of microspore embryogenesis,embryoids were induced from only four varieties,‘Y007',‘Y008',‘Y009'and ‘Y010'among all the test materials,the induced frequency of embryoid was 0. 80,1. 03,1. 97,and 1. 40 embryos per bud,respectively. There were significant differences of embryogenesis rates among different genotypes,‘Y014 ' cannot obtain embryoid though cultured with several times. low-frequency shaking with 60 r·min-1could promote the formation of embryoid,accelerate the embryogenesis,and increase the proportion of cotyledon-shaped embryos. The results of ploidy analysis showed that haploid,diploid and tetraploid can derived simultaneously from microspore,the ploidy variation were diversity and the rates of spontaneous chromosome doubling were 3. 2% to 92. 0%.