Observation of the radiosensitizing effect of DDP on Hela cell in vitro experiment

Objective To investigate the cytotoxic and radiosensitizing effects of cisplatin(DDP) on cervical carcinoma cell line Hela,and the effect relationship of radiation combined with DDP.Methods Under different concentrations of DDP and dose radiation,the inhibition on Hela cells was detected by MTT assay.When DDP and radiation were applied in combination,cell inhibition rate was detected by MTT assay,the rate curves were obtained,and then the condition of the synergistic effect was detected.Meanwhile,the radiosensitizing effect of DDP on Hela cell line was detected by clone formation assay,and the sensitizing enhancement ratio(SER) of different group cell line was measured with multi-target single hit model.Results The IC50 of DDP was 10μg/mL.When chemotherapy and radiation were used together on Hela at 12Gy dose radiation and 1-5μg/mL DDP on Hela cell line,the cytotoxic effect was obviously dose-and time-dependent within 48 hours;however,DDP showed no more cytotoxic effect when its concentration was over 5μg/mL.The SER of DDP was 1.320 under clone formation assay.When radiation dose was less than 10Gy,the drug and radiation had a synergistic effect.When radiation dose was over 10Gy,an additive effect or a subadditive effect was found.Conclusion Using DDP and radiation together has a significantly inhibitory effect on cervical cancer cell line Hela,and DDP has the best radiosensitizing dose.In order to ensure the synergistic effect,the radiotherapy sensitization chemotherapy should be utilized as early as possible.