Impact on growth of Hela cells by Cisplatin alone or combined with Vitamin C

Objective To investigate the effects of Vitamin C combined with Cisplatin(DDP) on human cervical carcinoma cell lines Hela.Methods After treatment of Hela cells with different dose of DDP or combination of VC,the inhibitory effect of Vitamin C and Cisplatin on cells was measured by MTT assay.The change of cell morphology was observed with laser confocal microscopy.Results DDP in different dose inhibited the cell proliferation,and had dose-effect relationship and inhibitory effect was enhanced when combined VC with DDP.Conclusion DDP can inhibit proliferation of Hela cells,the coordinative effect was observed by combining VC with DDP.