Effect of Cisplatin in Different Diluted Degrees on Cell Cycle and Apoptosis of SiHa Cell Line from Human Cervical Carcinoma

Objective To investigate the possible mechanism of anticancer action of Cisplatin (DDP) in vitro by studying the effect of DDP in different diluted degrees on the cell proliferation,apoptosis and cell cycle and of SiHa cell line from human cervical carcinoma.Methods The effect of DDP on the SiHa cell line from human cervical carcinoma in vitro was detected by WST-8 assay,the apoptosis morphologic feature was assessed by Hoechst 33258 staining,and the apoptosis and cell cycle arrest were analyzed by flow cytometry.Results DDP could significantly inhibit the proliferation of SiHa cell in partly a concentration-dependent and time-dependent manner.But the inhibitory effect was not evident when it was 0.01-1.0μg/ml DDP for 24h and 0.01-0.1μg/ml DDP for 48h (P0.05).Hoechst 33258 staining showed that typical appearance of cell apoptosis could be found in 5μg/ml DDP for 24h.The S cell cycle arrest of SiHa cell line could be found in 1μg/ml DDP for 24h,and the S cell proportion was (53.17±7.50)%,which was significantly different from that in the 5μg/ml DDP and control groups (P0.01),with no obviously apoptosis-induced effect by 1μg/ml DDP (P0.05).And the rates of early apoptosis,late apoptosis and sclerosis were (5.88±1.64)%,and (5.18±1.26)% respectively in 5μg/ml DDP for 24h,which were significantly higher than those in the 1μg/ml DDP and control groups(P0.05).Conclusion DDP could inhibit the proliferation in SiHa cells,but SiHa cells showed resistant to lower doses of DDP,which may be caused by S cell cycle arrest.The possible mechanism of anticancer action of higher doses of DDP in vitro was mainly inducing apoptosis.