Construction of Plant Expression Vector of Apple MdCKS Gene

Based on the sequences of the cyclin-dependent kinase genes in GenBank,the primers were designed for PCR with the restriction enzyme sites of XbaⅠ and SalⅠfrom the ORF of the MdCKS gene for PCR.The template was the cDNA of MdCKS from Fuji apple.The PCR fragment was firstly inserted into the pMD18-T Simple Vector,and then inserted into the plant expression vector pBI121 after sequenced.The plant expression vector of MdCKS gene was constructed successfully by resistance selection and sequence confirmation.The recombinant plasmid was transformed into Agrobacterium tumfaciens LBA4404 by electroporation for genetic transformation of tomato.