Expression Vector Construction of Strawberry FaEtr2 Gene

Two fragments of All-star strawberry FaEtr2 gene were cloned using All-star strawberry as materials.Sense and antisense expression vector were constructed for improving storage and transport varieties of strawberry through transgenic technology.According to the restriction enzyme sites of expression vector and cloning sequence of strawberry FaEtr2 gene,two pairs of primers containing restriction enzyme sites were designed and used to amplify sequenced plasmid.PCR products and the plasmid pBI221 were digested by the corresponding restricted enzymes respectively,and linked directionally.Then the sense and antisense expression vector can be designed.The constructed expression vector was transformed into Agrobacterium LBA4404 for the following-up research.