Construction of the over and antisense expression vector of CsLEA gene

According to the restriction enzyme sites of plant expression vector and sequence of CsLEA gene from Cleistogenes songorica,over expression vector was recombined by inserting the open reading frame( ORF) sequence to pBI121,and the antisense plant expression vector was constructed by ligating 480 bp sequence of CsLEA gene to pBI121 in the antisense orientation. Moreover,the recombined plasmid was transformed into Agrobacterium tumefaciens GV3101 by electroporation method. Using pollen-tube pathway,the plasmid was transformed into Arabidopsis thaliana and T1 positive plants were obtained by kanamycin resistance selection and PCR confirmation.