Construction of Eukaryotic Plant Expressing Vector Containing Isopentenyl Transferase Gene

A pair of primers was designed according to the sequences published by Genbank in order to amplify Isopentenyl Transferase Gene(ipt gene) of the plasmid in Agrobacterium tumefaciens(strain C58).The gene was amplified by PCR and cloned into pMD18-T vector,then was digested and inserted into the pBI121 vector,and transformed into the E.coli component DH5α.The expressing vector was indentified by PCR and then transformed into Agrobacterium tumefaciens EHA105.After the identification by PCR from EHA105 and pBI121-ipt vector,the specific expression vector of ipt was constructed successfully.The construction of recombinant pBI121-ipt plant expression vector provides a basis for the research on functions of ipt gene.