Effects of Pulse-activating Injection on NO and iNOS in Rats with Acute Hepatic Injury Induced by Paraquat

Objective: The variance of NO and iNOS on Paraquat induced rat acute hepatic injury was investigated, and the protection of pulse-activating injection on rat acute hepatic injury was approached. Methods: 50 Sprague-Dawley (SD) rats were randomLy divided into five experimental groups: blank group, negative control group, positive control group, pulse-activating injection low-dose group(LDG), pulse-activating injection high-dose group(HDG). Microscopical histopathological examinations were performed. In the mean time, the level of ALT, AST, MDA, SOD and GSH-Px in plasma were detected, and the activities of liver tissue homogenate NO and iNOS were measured. Results: Compared with that in negative control group, hemorrhage of liver of pulse-activating injection low-dose group were mitigated. ALT、AST、MDA、SOD and GSH-Px in plasma have statistical significance (P0.05, P0.01); and biological indexes of hepatic injury and the contents of NO and iNOS were significantly decreased (P0.05, P0.01). Conclusions: NO and iNOS could play an role in hepatic injury of poisoned rats, pulse-activating injection could ameliorate the hepatic injury and decrease the concentrations of NO and iNOS.