Empirical study on therapeutical effect of pulse-activating injection to acute lung injury induced by paraquat in rats

Objective:The variance of NO and iNOS of acute lung injury induced by paraquat on rats was investigated,and the protection of pulse-activating injection in acute lung injury on rat was approached.Methods:50 Sprague-Dawley(SD) rats were randomly divided into five experimental groups: blank group(NS),negative control group(PQ),positive control group(D),pulse-activating injection low-dose group(LDG),pulse-activating injection high-dose group(HDG).Macroscopic and histopathological examinations were performed and biological indexes were measured for the lung specimens.The indexes included lung wet weight/dry weight,the rats of neutrophils and protein content in the pulmonary alveolar lavage fluid,In the mean time,the activities of lung tissue homogenate NO and iNOS were measured.Results: Compared with that in negative control group,lung congestion and lung edema were mitigated.Lung wet weight/dry weight,rat of neutrophils,protein content were decreased(P0.05,P0.01);and biological indexes of lung injury and the contents of NO and iNOS were significantly decreased(P0.05,P0.01).Conclusion: NO and iNOS could play an important role in lung injury of poisoned rats.Pulse-activating injection could ameliorate the lung injury and decrease the concentrations of NO and iNOS.