ScholarLens Back to search
2007•Lishizhen Medicine and Materia Medica ResearchRequires access

Determination of Puerarinin Content in Guangxi Radix puerariae thomsonii by HPLC

Dongping Wei

Open publisher page 0 citations

Abstract

ObjectiveTo determine the content of puerarin from Guangxi Radix puerariae thomsonii by HPLC.Methods The isolation was conducted on a phenomenex gemini C18 column(250 rmm ×4.6 mm,5 μm),the mobile phase consisted of acetonitnle(A)-0.4% phosphoric and(B) in a gradient model,the flow rate wes 1.0 ml/min,the detection wavelength was 250 nm and the column temporature was 30℃.ResultsThe content of puerarin showed good linearity in the range of 800 ng~2400 ng,the regression equation was A=7.682×104C +5.885×104,r=0.999 3(n=5),and the average recovery was 99.49%,RSD was 0.75%(n=5).ConclusionThis method is simple,rapid,accurate and reliable.It can be used for quality control of Guangxi Radix puerariae thomsonii.

About this research paper

What this paper is about

ObjectiveTo determine the content of puerarin from Guangxi Radix puerariae thomsonii by HPLC.Methods The isolation was conducted on a phenomenex gemini C18 column(250 rmm ×4.6 mm,5 μm),the mobile phase consisted of acetonitnle(A)-0.4% phosphoric and(B) in a gradient model,the flow rate wes 1.0 ml/min,the detection wavelength was 250 nm and the column temporature was 30℃.ResultsThe content of puerarin showed good linearity in the range of 800 ng~2400 ng,the regression equation was A=7.682×104C +5.885×104,r=0.999 3(n=5),and the average recovery was 99.49%,RSD was 0.75%(n=5).ConclusionThis method is simple,rapid,accurate and reliable.It can be used for quality control of Guangxi Radix puerariae thomsonii.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

ObjectiveTo determine the content of puerarin from Guangxi Radix puerariae thomsonii by HPLC.Methods The isolation was conducted on a phenomenex gemini C18 column(250 rmm ×4.6 mm,5 μm),the mobile phase consisted of acetonitnle(A)-0.4% phosphoric and(B) in a gradient model,the flow rate wes 1.0 ml/min,the detection wavelength was 250 nm and the column temporature was 30℃.ResultsThe content of puerarin showed good linearity in the range of 800 ng~2400 ng,the regression equation was A=7.682×104C +5.885×104,r=0.999 3(n=5),and the average recovery was 99.49%,RSD was 0.75%(n=5).ConclusionThis method is simple,rapid,accurate and reliable.It can be used for quality control of Guangxi Radix puerariae thomsonii.

Key concepts: Chromatography, Puerarin, Radix (gastropod), High-performance liquid chromatography, Chemistry, Content determination, Phosphoric acid, Recovery rate

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Determination of Puerarinin Content in Guangxi Radix puerariae thomsonii by HPLC — Research Paper | ScholarLens