Study on the Extraction Technology of Polyphenol Substances from Grape Seeds

[Objective] The aim of the research was to provide the reference basis for the development and utilization of grape seeds in future.[Method] The concn.of extracting reagent,the material-liquid ratio,extracting time,extracting temperature and extracting times were selected to make the single factor experiments.On this basis,the orthogonal test was made to optimize the extraction technology of polyphenol substances from grape seeds.[Result] With the enhancing of volume fraction of methyl alcohol,the increasing of the material-liquid ratio,the increasing of temperature and the prolonging of the extracting time,polyphenol content in grape seeds was all increased.With the increase of extracting times,polyphenol content in grape seeds gradually was decreased.The effects of each factor on polyphenol content in grape seeds from high to low were as follows:extracting temperature volume fraction of methyl alcohol extracting time the material-liquid ratio.[Conclusion] The optimum extraction technology of polyphenol substances in grape seeds was as follows:the volume fraction of methyl alcohol of 70%,the material-liquid ratio of 1∶20,water bath at 90 ℃ and extracting for 30 min.