RP-HPLC determination of paeoniflorin,baicalin,palmatine hydrochloride and berberine hydrochloride in Kuntai Capsules

AIM: To develop an HPLC method for analyzing the contents of paeoniflorin,baicalin,palmatine hydrochloride and berberine hydrochloride in Kuntai Capsules(Praeparata Radix Rehmannial,Rhizoma Coptidis,Radix Paeoniae,Radix Scutellariae,Colla Asini corii,Poria).METHODS: Four compounds were determirned with an Agilent ZORBAX SB C18 column(4.6 mm × 250 mm,5 μm).Acetonitrile-0.4% phosphoric acid was used as the mobile phase at a flow rate of 1.0 mL/min.The mobile phase was in linear gradient elution.The detection wavelength was 230 nm.RESULTS: The linear ranges were within 0.016 58-0.331 6 μg(r = 0.999 9) for paeoniflorin,0.059 80-1.196 0 μg(r = 0.999 9)for baicalin,0.012 91-0.258 2 μg(r = 0.999 9)for palmatine hydrochloride,0.041 98-0.839 6 μg(r = 0.999 9) for berberine hydrochloride.The average recoveries (n = 6) were 98.91% (RSD = 1.29% ),99.20% (RSD = 0.92% ),97.86% (RSD = 0.89% ),98.63% (RSD = 0.62% ),respectively.CONCLUSION: The method is simple,reliable,sensitive and can be applied to having controls over the preparation.