Determination of paeoniflorin,palmatine hydrochloride and berberine hydrochloride in Changji'an Preparation by HPLC

AIM To establish an HPLC method for determining the contents of paeoniflorin,palmatine hydrochloride and berberine hydrochloride in Changji'an Preparation(Paeoniae Radix alba,Coptidis Rhizoma,Atractylodis macrocephalae Rhizoma,Saposhnikoviae Radix,etc.).METHODS HPLC was used.The Kromat universal C18 column was adopted with acetonitrile-0.1% phosphoric acid solution(15∶ 85) for the determination of paeoniflorin and the clipse XDB-C18 column was adopted with acetonitrile-0.05 mol/L potassium dihydrogen phosphate(50∶ 50)for the determination of palmatine hydrochloride and berberine hydrochloride.The flow rate was 1.0 mL/min and the detection wavelengths were set at 230 nm and 345 nm,respectively.The column temperatures were maintained at 25 ℃,20 ℃,respectively.RESULTS The separations of paeoniflorin,palmatine hydrochloride and berberine hydrochloride peaks were good.The calibration curves were linear in the ranges of 0.102 02-0.816 16 μg for paeoniflorin,0.027 9-0.139 4 μg for palmatine hydrochloride and 0.111 5-0.557 5 μg for berberine hydrochloride.The average recoveries were 99.82%,101.06% and 100.23%,respectively.The RSDs were 1.71%,1.97% and 1.92%(n=9),respectively.The precision,stability and reproducibility were good.CONCLUSION This method is simple,efficient,and can guide the research in future.