Effects of Fermented Pu-erh Tea,Tieguanyin Tea and Black Tea on Expression of Hepatocyte IGF-1 in Rats with Non-alcoholic Fatty Liver Disease

Objective To approach the effects of the fermented Pu-erh tea,Tieguanyin tea and Black tea on the expression of hepatocyte IGF-I in rats with non-alcoholic steatosis induced by high fat diet.Methods Wistar rats were randomly divided into control group,hyperlipidemia model group,medicinal group,and low,medium and high dose groups(0.5,1.0,2.0 g/kg·bw) of fermented Pu-erh tea,Tieguanyin tea and Black tea individually.All rats were sacrificed after 35 days' intragastric administration with the aqueous extracts of tested teas.The serum levels of TC and TG and the activities of ALT and AST were determined.The expression quantity of insulin-like growth factor I(IGF-I) in hepatocyte was detected by RT-PCR method.Results Compared to hyperlipidemia model group,the serum levels of TC were significantly decreased(P0.05,P0.01)in medicinal group,medium and high dose groups of fermented Pu-erh tea,low and medium dose groups of Tieguanyin tea,and as well as low and medium dose groups of Black tea;the serum levels of TG were significantly decreased(P0.05,P0.01)in medicinal group,low,medium and high dose groups of fermented Pu-erh tea,low and medium dose groups of Tieguanyin tea,and as well as low,medium and high dose groups of Black tea.Compared to hyperlipidemia model group,the serum levels of AST were significantly decreased(P0.05,P0.01) in low,medium and high dose groups of fermented Pu-erh tea,low and medium dose groups of Tieguanyin tea,and as well as high dose group of Black tea;the serum levels of ALT were significantly decreased(P0.05,P0.01) in low,medium and high dose groups of fermented Pu-erh tea,and as well as low and high dose groups of Black tea;the expression of IGF-I were increased in high does group of fermented Pu-erh tea and medium does group of Tieguanyin tea.Conclusion Fermented Pu-erh tea,Tieguanyin tea and Black tea can have different degrees of positive effects on the expression of IGF-I in rat non-alcoholic steatosis model.And Fermented Pu-erh tea has the most obvious effect.