Effect of Fermented Pu-erh Tea Powder,Dark Tea Powder and Liupu Tea on Hepatocytic CYP2E1 Expression in Rats with Experimental Nonalcoholic Fatty Liver

Background: CYP2E1 is an ethanol-inducible cytochrome P450 enzyme that plays an important role in thetwo hits hypothesis of nonalcoholic fatty liver disease by inducing lipid peroxidation.Aims: To investigate the auxiliary protective effect of Fermented Pu-erh Tea powder,Dark Tea powder and Liupu Tea on rats with experimental nonalcoholic fatty liver(NAFL) from the perspective of improving oxidative stress.Methods: One hundred and twenty Wistar rats were divided into normal control group,hyperlipidemia model group,hypolipidemic agent group and low,moderate and high dose(0.5,1.0 and 2.0 g / kg) Fermented Pu-erh Tea powder,Dark Tea powder and Liupu Tea groups.NAFL was induced by high fat diet.Rats in experimental groups were administered intragastrically with different tea infusion for 35 days.All rats were sacrificed after 35 days feeding,several hepatic antioxidant indices were measured,the hepatocytic CYP2E1 mRNA expression was detected by real time PCR,and hepatic histopathological changes were also observed.Results: Eligible doses of Fermented Pu-erh Tea powder and Liupu Tea significantly decreased the concentration of MDA and expression of CYP2E1 mRNA,and increased the activities of SOD and GSH-Px in liver tissue of hyperlipidemic rats(P all 0.05).Under microscopy,rats in high dose Liupu Tea group presented mild fatty liver and those in high dose Fermented Pu-erh Tea powder group and Dark Tea powder group presented moderate fatty liver.Conclusions: Fermented Pu-erh Tea powder and Liupu Tea have positive effects on antioxidant indices,CYP2E1 gene expression and histopathology of liver tissue in NAFL model rats,which denotes an auxiliary protective effect on NAFLD,and Liupu Tea is more effective than Fermented Pu-erh Tea powder.