Clinical estimation of time resolved fluorescence immunoassay on detection of serum markers of hepatitis B virus

Objective:To estimate the clinical value of the time resolved fluoescence immunoassay(TRFIA) in detecting serum markers of hepatitis B virus(HBV-M).Methods:We used the methods of TRFIA and ELISA to measure the HBV-M of 115 serum samples from control subjects and 210 serum samples from patients respectively.Results:There were no discrepancy in control subject and patients using the methods of TRFIA and ELISA to measure HBsAg;TRFIA has a higher sensitivity to HBsAb.TRFIA was able to detect excessively low or high concentraction of HBsAb.Also it had a higher speciality to HBeAg,while ELISA had a higher rate of false positive relatively.There was statistical difference between ELISA and TRFIA in detecting HBeAb and HBcAb.Conclusion:TRFIA has a higher sensitivity and speciality in detecting HBV-M,which could reflect the HBV infection condition indirectly and provide a reliable guide on injecting HBV vaccine.Monitoring the developmemt of HBV-M content provides a good reflection on our body's reaction to virus infecting,which can be used as the evidence for observing clinical course of disease and curative effect.