Application of time-resolved fluorescence immunoassay on detection of markers of hepatitis B

Objective To investigate the clinical value of time-resolved fluorescence immunoassay( TRFIA) on detec-ting five makers of hepatitis B by comparing the results of TRFIA and enzyme-linked immunoassay assay( ELISA) on detection of HBV markers. Methods The accuracy and the repeatability for the necessary reagent box was measured by TRFIA. Five markers of hepatitis B of 235 serum samples were measured by TRFIA and ELISA simultaneously. Results Five makers of hepatitis B was measured with good accuracy and repeatability by TRFIA. There was no remarkable significance between ELISA and TRFIA in detecting HBsAg and HBeAg( P 0. 05). But there was significant difference between ELISA and TRFIA in detecting HbsAb、HbeAb and HBcAb( P 0. 01). Compared with ELISA in HBV-M determination,the detection ratio of the mod-el( HbsAb、HbeAb、HbcAb) by TRFIA were significantly higher( P 0. 05) ,but others had no significant difference( P 0. 05). Conclusion TRFIA is more sensitive and higher specific than ELISA. It is an ideal quantitative detection method. TR-FIA can provide comprehensive and dynamic monitoring for the diagnosis、treatment and prognosis of hepatitis B thought the detection of HBV markers,and it also can provide reliable basis for vaccination.