Cloning and sequence analysis of full-length cDNA encoding cyclophilin in Vernicia fordii

Cyclophilins are ubiquitously distributed in organisms,plant cyclophilins play an important roles in plant biology,stress response and anti-pathogen immunity.According to the part sequence of cyclophilin gene in seed EST library from Vernicia fordii,a fulllength cDNA encoding cyclophilin obtained with the method of RACE and RT-PCR.The length of Vernicia fordii cyclophilin is 681bp,contained a complete CDS of 519bp encoding 172 amino acids,named vf_cyp.The bioinformatics analysis indicated that the pI and MW of amino acids encoded by vf_cyp were predicted to be 8.68 and 18038.5Da respectively.The prediction result indicated membrane spaning domain and signal peptide were not exist in the protein,the secondary structure of the vf_cyp are β-sheet(36.05%) and Random coil(52.91%) primarily,the protein had one peptidyl-prolyl cis-trans isomerase binding domain,and containing the plant cyclophilins unique 7 amino acid insertion sequence KS/MGKPLH,the amino acid sequence of Vernicia fordii Cyclophilin showed similarities of 94%,94%,93% with those from Citrus sinensis,Citrus hybrid cultivar,Populus trichocarpa,respectively.