Study on the Cloning and Sequence Analysis of FADX Gene from Vernicia fordii

[Objective] The research aimed to clone full-length cDNA of FADX of Vernicia fordii seeds.The bioinformatic analysis of this gene was done,and provided reference for the further research of the function of gene.[Method] Total RNA were successfully extracted by developing Vernicia fordii seeds with improved TRIzoL method.Primer was designed according to the FADX sequences recorded in Genbank.Full-length cDNA of FADX was obtained by cloning it with RT-PCR method.Bioinformatic analysis were done by using the software of Antheprot and Interproscan.[Result] The length of 5' side and 3' side unencoded area's sequences were 13 bp and 47 bp,contained an opening reading frame from 14 bp to 1 174 bp which encoded a polypeptide of 386 amino acids,and contained a specific fattic acid dehydrogenase structural domain.Relative molecular weight was 44 343.0,pI was 8.33,and the calculation of secondary structure showed that the protein contained 29% in α-helix,32% in β-pleated sheet,and 6% inβ-return.[Conclusion] This protein had the property of fattic acid dehydrogenase.