Cloning and sequence analysis of BC gene from Vernicia fordii

With nearly ripe Puputung tree seeds as the tested materials, the pri mers were designed according to the results of the transcriptome sequencing analysis of Vernicia fordii, and a full-length c DNA of BC gene was cloned by RT-PCR technique from nearly ripe seed in Vernicia fordii. The c DNA sequence length of BC gene is 1605 bp, encoding 534 amino acids. BC protein's isoelectric point(p I) is 7.98 and the molecular mass of the protein is 58262.0 Da, it's stability factor is 38.13. According to bioinformatics analysis, the protein bears two trans-membran e domains, and is a unstable protein. There is a ATP-grasp fold profile in BC. There are 16 alpha helixes in the tertiary structure and there is a hollow concave structure in the central of this protein.